Blood components

Pooled leukoreduced platelets

DEFINITION

These are platelets from several units of total blood.

HOW IT IS OBTAINED

By centrifuging a unit of total blood, the leucoplatelet layer is separated and obtained in a bag. This product is centrifuged again to concentrate the platelets, which are then filtered to eliminate most of the leucocytes. Finally, several (generally five) of these units are mixed and a solution is added (0.29% sodium citrate 2 H2O, 0.40% sodium acetate 3 H2O and 0.67% sodium chloride) to enhance the preservation of the platelets.

COMPOSITION AND CHARACTERISTICS

Volume: the average volume is 305.2 ± 35.3 ml.
Platelet content : more than 2.5 x 1011.
Leucocytes: less than 1 x 106
Ph (after five days' preservation): the average Ph at the end of the preservation period is 6.92 ± 0.17. In all cases it remains between 6.4 and 7.2.

ANALYSIS RESULTS

  • ABO group and Rh factor.
  • Irregular antibodies: negative.
  • Surface antigen of the hepatitis virus B (HBs Ag): negative.
  • Antibodies against the hepatitis C virus (Anti-HCV): negative.
  • Antibodies against the Immunodeficiency virus (Anti-HIV 1+2): negative.
  • Syphilis serology: negative.
  • NAT of HCV (PCR): negative.
  • NAT of HIV (PCR): negative.
  • NAT of VHB (PCR): negative.
  • Anti-Trypanosoma cruzi antibodies: negative (only carried out on donors with a risk of being carriers).

RECIPIENT

Collapsible plastic bags.

IDENTIFICATION

With labels containing the following data: name of the centre, type of product, ABO group and Rh (D) factor, unit number, date of extraction, expiry date, results of analysis, type of anticoagulant, preservation and administration conditions.

PRESERVATION

At 22ºC ± 2ºC in continual movement.

EXPIRY DATE

5 days.

HANDLING

The platelet pools can be irradiated.