Clinical information
Diagnostic Utility:
Identification of anti-HLA antibodies allows us to detect the presence of donor-specific anti-HLA antibodies (DSA) in:
- Patients candidates for hematopoietic stem cell transplantation.
- Patients candidates for solid organ transplantation.
- Platelet refractoriness
Identification of anti-HLA antibodies allows us to detect the presence of recipient-specific anti-HLA antibodies in:
- Suspected Transfusion-Related Acute Lung Injury (TRALI).
Method:
Solid-phase assay (Luminex).
The serum to be analyzed is incubated with microspheres presenting HLA antigens on their surface. A human anti-IgG antibody conjugated with phycoerythrin is added, allowing visualization of the binding of anti-HLA antibodies present in the serum using the LUMINEX instrument.
Reference Values
During identification, the specificities of anti-HLA antibodies are determined. Results indicate the specificities of the detected antibodies and their mean fluorescence intensity (MFI), for example: anti-HLA-A*02:01 antibody (MFI 10,000). A positive result is considered when the MFI is greater than 750 and exceeds the cutoff value for the MFI/LRA ratio.
Diagnostic Algorithm:
Following the diagnostic algorithm, if the result of the anti-HLA antibody search is positive (for class I and/or II), identification of anti-HLA antibodies (for class I and/or II) is carried out to determine the specificities of these antibodies.
*Presence of DSA reported
Turnaround Time:
15 working days.
Specimen information
Sample: Serum
Tube: 8.5 ml separator gel tube
Minimum volume:
- Blood: 500µl.
- Serum: 100 µl.
Stability:
- Refrigerated: 2 days.
- At -20ºC: from day 3 onwards.
Transport instructions:
If it is a blood sample, preferably at room temperature.
If it is a serum sample, preferably refrigerated.
Reason for rejection: Highly hemolyzed sample
Administrative information
BST Code: LRD2017
Test Description: Identification of anti-HLA class I antibodies
Synonyms: Not applicable
Section: Histocompatibility and Immunogenetics
BST Rate: Check the updated rates here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).
Quality
BST holds ISO 9001, ISO 14001, and OSHAS 18001 quality certifications, as well as the European Excellence 500+ seal. BST is accredited by CAT, JACIE-FACT, FACT-NETCORD, and EFI, and complies with Good Manufacturing Practice (GMP) and Good Distribution Practice (GDP) guidelines.