Clinical information
Diagnostic Utility:
Identification of anti-HLA antibodies allows us to detect the presence of donor-specific anti-HLA antibodies (DSA) in:
- Patients candidates for hematopoietic stem cell transplantation.
- Patients candidates for solid organ transplantation.
- Platelet refractoriness
Identification of anti-HLA antibodies allows us to detect the presence of recipient-specific anti-HLA antibodies in:
- Suspected Transfusion-Related Acute Lung Injury (TRALI).
Method:
Solid-phase assay (Luminex).
The serum to be analyzed is incubated with microspheres presenting HLA antigens on their surface. A human anti-IgG antibody conjugated with phycoerythrin is added, allowing visualization of the binding of anti-HLA antibodies present in the serum using the LUMINEX instrument.
Reference Values
During identification, the specificities of anti-HLA antibodies are determined. Results indicate the specificities of the detected antibodies and their mean fluorescence intensity (MFI), for example: anti-HLA-A*02:01 antibody (MFI 10,000). A positive result is considered when the MFI is greater than 750 and exceeds the cutoff value for the MFI/LRA ratio.
Diagnostic Algorithm:
Following the diagnostic algorithm, if the result of the anti-HLA antibody search is positive (for class I and/or II), identification of anti-HLA antibodies (for class I and/or II) is carried out to determine the specificities of these antibodies.
*Presence of DSA reported
Turnaround Time:
15 working days.
Specimen information
Sample: Serum
Tube: 8.5 ml separator gel tube
Minimum volume:
- Blood: 500µl.
- Serum: 100 µl.
Stability:
- Refrigerated: 2 days.
- At -20ºC: from day 3 onwards.
Transport instructions:
If it is a blood sample, preferably at room temperature.
If it is a serum sample, preferably refrigerated.
Reason for rejection: Highly hemolyzed sample
Administrative information
BST Code: LRD2017
Test Description: Identification of anti-HLA class I antibodies
Synonyms: Not applicable
Section: Histocompatibility and Immunogenetics
BST Rate: Check the updated rates here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).