Clinical information
Diagnostic Utility:
The HLA-B27 allelic family is part of the HLA class I locus B, comprising 26 alleles grouped under the same serological specificity. Regarding its average population frequency, in the Caucasian population it can be said to vary between 3% and 8%. Despite its low frequency, the interest in this allelic family lies in the strong association it maintains with a group of rheumatic diseases, the spondyloarthropathies among which Ankylosing Spondylitis (AS) can be highlighted as the most strongly associated and important disease. Approximately 90% of patients with this type of disease are B*27 positive.
Method:
Genotyping of the HLA-B27 antigen by real-time PCR with FRET hybridization probes.
Reference Values
The possible results are: B*27 positive or B*27 negative.
Diagnostic Algorithm:
Not applicable
Turnaround Time:
10 working days.
Specimen information
Sample: Whole Blood
Tube: EDTA K3 4 ml Tube
Minimum volume: 500µl.
Stability:
- In refrigeration: 2 weeks.
- At -20ºC: from 2 weeks onwards.
Transport instructions: Preferably at room temperature
Reason for rejection: Coagulated sample
Administrative information
BST Code: LRD2001
Test Description: HLA-B27
Synonyms: Not applicable
Section: Histocompatibility and Immunogenetics
BST Fee: Check the updated fees here.
Profiles: Not applicable.
References
R. Faner, N. Casamitjana, R. Colobran, A. Ribera, R. Pujol, E. Palou, M. Juan. "HLA-B27 genotyping by fluorescent resonance emission transfer (FRET) probes in real-time PCR" Human Immunology. 65(10): 826-838. 2004.
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).