ELISPOT Lymphocytes T virus-specific
Code: CEL027
Type: Control de qualitat de medicaments de teràpia avançada
Clinical information
Diagnostic Utility:
The ELISPOT technique is used for the detection of interferon-gamma secreting T cells in the presence of viral antigens such as PepTivator®CMV pp65, PepTivator®CMV IE-1, PepTivator®AdV5 Hexon, PepTivator®EBV Consensus, PepTivator®EBV EBNA1, PepTivator®BKV LT, PepTivator®BKV VP1. This determination is used for donor assessment and patient monitoring in the research or healthcare setting.
Method:
ELISPOT (Enzyme Linked Immuno SPOT assay) is a quantitative technique based on enzyme-linked immunosorbent assay designed to count cytokine-secreting cells.
Reference Values
The conformity criteria are as follows:
| Conformity Criteria | ||
|---|---|---|
| Viruses to be tested | Cytomegalovirus (CMV) | ≥20 spots |
| Adenovirus (ADV) | ≥10 spots | |
| Epstein-Barr (EBV) | ||
| BK | ||
| Controls | Positive | ≥20 spots |
| Negative | No spots present | |
Diagnostic Algorithm:
Not applicable.
Turnaround Time:
2 days
Specimen information
Sample: Peripheral Blood.
Tube: Lithium Heparin Tube.
Minimum volume: 20 mL
Stability:
- At room temperature: 24 hours
Transport instructions: Peripheral blood at room temperature.
Reasons for rejection: Sample highly hemolyzed or with clots.
Administrative information
BST Code: CEL027
Test Description: ELISPOT Virus-specific T lymphocytes
Synonyms: ELISPOT
Section: Cellular Laboratory
BST Rate: Check the updated rates here.
Profiles:
The CEL027 ELISPOT Virus-specific T lymphocytes test is also performed within the following profiles:
| Profile Name | Profile Code |
|---|---|
| VIROTCELL Donor | C38 |
| VIROTCELL Recipient | C39 |
References
No aplica