Gamma interferon secretion assay

Clinical information

Diagnostic Utility:

Test that determines the presence of specific T lymphocytes that secrete interferon gamma against viruses such as Cytomegalovirus, Adenovirus, Epstein Barr, and BK virus. It is a test used to determine if a donor has specific lymphocytes against a particular virus and thus evaluate if they are suitable as a donor for immunotherapy treatments.

Method:

Flow cytometry

Reference Values

To determine the presence of specific T lymphocytes, there must be a minimum percentage of these.

Diagnostic Algorithm:

Not applicable.

Response Time:

1 day.

Specimen information

Sample: Peripheral blood
Tube: Heparin Lithium Tube
Minimum volume:  50mL
Stability: 24h at room temperature
Transport instructions: Room temperature
Reason for rejection: Sample highly hemolyzed or with clots.

Administrative information

BST Code: CEL026
Test Description: Gamma interferon secretion assay
Synonyms: IFNg secretion
Section: Cell Laboratory
BST Rate: Check the updated rates here.

Profiles:

This test should always be requested together with the test CEL003 Complete blood countº. It is also included in the following profile:

References

• NCF Parte IV: Directrices sobre normas de correcta fabricación específicas para Medicamentos de Terapia Avanzada.
• Detection of cytokine secreting cells from whole blood. Miltenyi Biotech. 140-000-662.02.