CD34+/CD133+ Expression
Code: CEL017
Type: Control de qualitat de medicaments de teràpia avançada
Clinical information
Diagnostic Utility:
Test that quantifies
- cells positive for the CD34 membrane receptor (surface antigen of hematopoietic stem cells)
- cells positive for the CD133 membrane receptor (found in hematopoietic stem cells and endothelial cells)
and determines cellular viability.
It is a determination used as a quality control of products from positive selections of CD133 cells for the treatment of diseases such as Asherman's Syndrome or by IPS cells.
Method:
Flow cytometry
Reference Values
The results are quantitative. CD34 and CD133 concentrations can vary greatly depending on the type of sample/product.
Diagnostic Algorithm:
Not applicable.
Response Time:
1 day.
Specimen information
Sample: Peripheral blood, umbilical cord blood, bone marrow, apheresis.
Tube: EDTA K3 tube (peripheral blood) or Additive-free tube (anticoagulated samples).
Minimum volume: 100 µL
Stability:
- At room temperature: 1 day
- In refrigeration: 1 day
- Cryopreserved between -156oC and -196oC: 20 years
Transport instructions: Room temperature
Reason for rejection: Sample with DNA or precipitates
Administrative information
BST Code: CEL017
Test Description: CD34+/CD133+ expression
Synonyms: CD133+
Section: Cellular Laboratory
BST Fee: Check the updated fees here.
Profiles: This test must always be requested together with test CEL007. It is also carried out within the following profiles:
| Profile Name | Profile Code |
|---|---|
| INITIAL SEL. CD133 | CE33 |
| POSTNIGHT SEL. CD133 | CE34 |
| FR SEL. CD133+ | CE35 |
| FR SEL. CD133- | CE36 |
References
Cervelló et al. Human CD133Dbonemarrow-derived stem cells promoteendometrial proliferation in a murinemodel of Asherman syndrome; Fertility and Sterility, Volume 104, Issue 6, December 2015, Pages 1552-1560.e3
Santamaria X, et al. Autologous cell therapy with CD1331bone marrow-derived stem cells forrefractory Asherman's syndrome andendometrial atrophy: a pilot cohortstudy; Human Reproduction, Vol.31, No.5 pp. 1087-1096, 2016
Quality
BST holds ISO 9001, ISO 14001, and OSHAS 18001 quality certifications, as well as the European Excellence 500+ seal. BST is accredited by CAT, JACIE-FACT, FACT-NETCORD, and EFI, and complies with Good Manufacturing Practice (GMP) and Good Distribution Practice (GDP) guidelines.