Viability Blue Belly
Clinical information
Diagnostic Utility:
This test quantifies cellular viability in thawed clinical samples of umbilical cord blood and thawed or post-thaw hematopoietic progenitor samples for clinical use. It is also used to evaluate cellular viability in cell culture samples for research projects.
Method:
Manual count in a Neubauer chamber.
Reference Values
The results are quantitative. The expected values are as follows:
| Sample | Trypan Blue Viability (%) |
|---|---|
| UCB (thawed) | ≥40 |
| Hematopoietic progenitors | ≥70 |
| Cell cultures (fresh) | ≥70 |
Diagnostic Algorithm:
Not applicable.
Turnaround Time:
1 day.
Specimen information
Sample: Thawed umbilical cord blood; thawed bone marrow/apheresis or post-night and cell cultures.
Tube: Additive-free tube
Minimum volume: 100 µL
Stability:
- Cell culture sample: 1 day
- Thawed/post-night samples: 1 hour
Transport instructions: Culture and post-night samples at room temperature; thawed samples with refrigerated transport.
Reason for rejection: Sample with clots.
Administrative information
BST Code: CEL013
Test Description: Determination of cell viability with Trypan blue.
Synonyms: BT
Section: Cellular Laboratory
BST Rate: Check the updated rates here.
Profiles:
The CEL013 test Trypan Blue Viability can be requested alone. It is also performed within the following profiles:
| Profile Name | Profile Code |
|---|---|
| SCU POST-WASH | CEL5 |
| SCU SE | CEL1 |
| AFE AUTO POSTNIGHT | CEL15 |
| TUB A AUTO | CEL7 |
| TUB A ALLO | CEL8 |
| TUB B/C AUTO | CEL9 |
| TUB B/C ALLO | CEL10 |
| TUB A/B/C ALLOQUOT/ CART | CEL11 |
References
Strober W; Curr Protoc Immunol. 2015 Nov 2;111:A3.B.1-A3.B.3.