Clinical information
Diagnostic Utility:
Identification of anti-HLA antibodies allows us to detect the presence of donor-specific anti-HLA antibodies (DSA) in:
- Patients being considered for hematopoietic stem cell transplantation.
- Patients being considered for solid organ transplantation.
- Platelet refractoriness.
Identification of anti-HLA antibodies allows us to detect the presence of recipient-specific anti-HLA antibodies in:
- Suspected Transfusion-Related Acute Lung Injury (TRALI).
Method:
Solid-phase assay (Luminex).
The serum to be analyzed is incubated with microspheres that present HLA antigens on their surface. A human anti-IgG antibody conjugated to phycoerythrin is added, allowing visualization of the binding of anti-HLA antibodies present in the serum using the LUMINEX device.
Reference Values:
In the identification, the specificities of the anti-HLA antibodies are determined. The results indicate the specificities of the detected antibodies and their mean fluorescence intensity (MFI), for example: anti-HLA-DRB1*03:01 antibody (MFI 10,000). It is considered positive when the MFI is greater than 750 and exceeds the cut-off value of the MFI/LRA ratio.
Diagnostic Algorithm:
Following the diagnostic algorithm, if the result of the anti-HLA antibody screening is positive (for class I and/or II), identification of anti-HLA antibodies (for class I and/or II) is performed to determine the specificities of these antibodies.
*Presence of DSA reported
Turnaround Time:
15 working days.
Specimen information
Sample: Whole Blood
Tube: EDTA K3 4 ml Tube
Minimum Volume: 500µl.
Stability:
- In refrigeration: 2 days.
- At -20ºC: from day 3 onwards.
Transport Instructions:
- If it is a blood sample, preferably at room temperature.
- If it is a serum sample, preferably refrigerated.
Reason for Rejection: Highly hemolyzed sample
Administrative information
BST Code: LRD2059
Test Description: Identification of anti-HLA class II antibodies
Synonyms: Not applicable.
Section: Histocompatibility and Immunogenetics
BST Rates: Check the updated rates here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).
Quality
BST holds ISO 9001, ISO 14001, and OSHAS 18001 quality certifications, as well as the European Excellence 500+ seal. BST is accredited by CAT, JACIE-FACT, FACT-NETCORD, and EFI, and complies with Good Manufacturing Practice (GMP) and Good Distribution Practice (GDP) guidelines.