Clinical information
Diagnostic Utility:
Identification of anti-HLA antibodies allows us to detect the presence of donor-specific anti-HLA antibodies (DSA) in:
- Patients being considered for hematopoietic stem cell transplantation.
- Patients being considered for solid organ transplantation.
- Platelet refractoriness.
Identification of anti-HLA antibodies allows us to detect the presence of recipient-specific anti-HLA antibodies in:
- Suspected Transfusion-Related Acute Lung Injury (TRALI).
Method:
Solid-phase assay (Luminex).
The serum to be analyzed is incubated with microspheres that present HLA antigens on their surface. A human anti-IgG antibody conjugated to phycoerythrin is added, allowing visualization of the binding of anti-HLA antibodies present in the serum using the LUMINEX device.
Reference Values:
In the identification, the specificities of the anti-HLA antibodies are determined. The results indicate the specificities of the detected antibodies and their mean fluorescence intensity (MFI), for example: anti-HLA-DRB1*03:01 antibody (MFI 10,000). It is considered positive when the MFI is greater than 750 and exceeds the cut-off value of the MFI/LRA ratio.
Diagnostic Algorithm:
Following the diagnostic algorithm, if the result of the anti-HLA antibody screening is positive (for class I and/or II), identification of anti-HLA antibodies (for class I and/or II) is performed to determine the specificities of these antibodies.
*Presence of DSA reported
Turnaround Time:
15 working days.
Specimen information
Sample: Whole Blood
Tube: EDTA K3 4 ml Tube
Minimum Volume: 500µl.
Stability:
- In refrigeration: 2 days.
- At -20ºC: from day 3 onwards.
Transport Instructions:
- If it is a blood sample, preferably at room temperature.
- If it is a serum sample, preferably refrigerated.
Reason for Rejection: Highly hemolyzed sample
Administrative information
BST Code: LRD2059
Test Description: Identification of anti-HLA class II antibodies
Synonyms: Not applicable.
Section: Histocompatibility and Immunogenetics
BST Rates: Check the updated rates here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).