Clinical information
Diagnostic Utility:
HLA typing at low resolution allows us to determine the HLA typing at the allele group level (2 digits) for:
- Family members of patients who are candidates for hematopoietic stem cell transplantation.
- Confirmation of HLA typing for patients who are candidates for hematopoietic stem cell transplantation and their donors (both related and unrelated).
- Patients who are candidates for solid organ transplantation.
- Verification of umbilical cord blood units.
- Study of the maternal sample from umbilical cord blood units (maternal haplotype).
Method:
PCR-SSO for the HLA-B locus.
Exons 2 and 3 of the HLA-B genes are amplified by PCR using biotinylated primers. The amplified products are hybridized with specific oligonucleotide probes immobilized on microspheres. Subsequently, the addition of a streptavidin-PE buffer allows visualisation of the hybridization pattern using the LUMINEX system.
Reference Values
With low-resolution typing, two results are obtained for each HLA gene, for example: HLA-B*18 B*27..
Diagnostic Algorithm:
Not applicable.
Turnaround Time:
20 working days.
Specimen information
Sample: Whole blood
Tube: EDTA K3 4 ml tube
Minimum volume: 500µl.
Stability:
- In refrigeration: 2 weeks.
- At -20ºC: from 2 weeks onwards.
Transport instructions: Preferably at room temperature
Reason for rejection: Clotted sample
Administrative information
BST Code: LRD2004
Test Description: HLA-B low resolution typing
Synonyms: HLA-B.
Section: Histocompatibility and Immunogenetics
BST Fee: Check the updated fees here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).