Clinical information
Diagnostic Utility:
HLA typing at low resolution allows us to determine HLA typing at the allelic group level (2 digits) for:
- Family members of patients candidates for hematopoietic progenitor cell transplantation.
- Confirmation of HLA typing for patients candidates for hematopoietic progenitor cell transplantation and their donors (both related and unrelated).
- Patients candidates for solid organ transplantation.
- Verification of units of umbilical cord blood.
- Study of the maternal sample of umbilical cord blood units (maternal haplotype).
Method:
PCR-SSO for the HLA-A locus.
Exons 2 and 3 of the HLA-A gene are amplified by PCR using biotinylated primers. The amplified products are hybridized with specific oligonucleotide probes immobilised on microspheres. Subsequently, the addition of streptavidin-PE buffer allows visualisation of the hybridization pattern using the LUMINEX system.
Reference Values
With low-resolution typing, two results are obtained for each HLA gene, for example: HLA-A*02 A*26.
Diagnostic Algorithm:
Not applicable.
Turnaround Time:
20 working days.
Specimen information
Sample: Whole Blood
Tube: EDTA K3 4 ml tube
Minimum volume: 500µl.
Stability:
- In refrigeration: 2 weeks.
- At -20ºC: from 2 weeks onwards.
Transport instructions: Preferably at room temperature
Reason for rejection: Clotted sample
Administrative information
BST Code: LRD2003
Test Description: HLA-A low resolution typing
Synonyms: HLA-A
Section: Histocompatibility and Immunogenetics
BST Fee: Check the updated fees here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).