Quantification/viability CD3 and CD45

Clinical information

Diagnostic utility:

This test quantifies:

• cells positive for the CD3 membrane receptor (T lymphocyte antigen)
• cells positive for CD45 (common leukocyte antigen)

and determines cellular viability. It is a determination used as a quality control for units of umbilical cord blood and products for hematopoietic progenitor transplantation.

Method: 

Flow Cytometry.

Reference values

The results are quantitative. CD3 and CD45 concentrations can vary greatly depending on the type of sample/product and whether they come from a healthy donor or a patient. As for cellular viability, the expected values are as follows:

Diagnostic algorithm:

Not applicable.

Turnaround time:

1 day.

Specimen information

Sample: Peripheral blood, umbilical cord blood, bone marrow, apheresis.
Tube: EDTA K3 tube (peripheral blood) or tube without additive (anticoagulated samples).
Minimum volume: 100 µL.
Stability:

• At room temperature: 1 day
• In refrigeration: 1 day
• Cryopreserved between -156^oC and -196^oC: 20 years

Transport instructions: Preferably peripheral blood at room temperature; umbilical cord blood, bone marrow, apheresis refrigerated.

Reason for rejection: Sample highly hemolyzed or with clots.

Administrative information

BST Code: CEL006
Test Description: Quantification and viability of CD3 and CD45 cells.
Synonyms: R/V CD3 and CD45.
Section: Cellular Laboratory
BST Rate: Check updated rates here.

Profiles:

Test CEL006 Quantification/viability CD3 and CD45 can be requested individually. It is also performed within the following profiles:

References

No aplica