Clinical information
Diagnostic Utility:
HLA typing at low resolution allows us to determine the HLA typing at the allelic group level (2 digits) of:
- Family members of patients who are candidates for hematopoietic stem cell transplantation.
- Confirmation of HLA typing for patients who are candidates for hematopoietic stem cell transplantation and their donors (both related and unrelated).
- Patients who are candidates for solid organ transplantation.
- Verification of umbilical cord blood units.
- Study of maternal samples from umbilical cord blood units (maternal haplotype).
Method:
PCR-SSO for the HLA-DRB1 locus.
Exons 2 and 3 of the HLA-DRB1 genes are amplified by PCR using biotinylated primers. The amplified products are hybridised with specific oligonucleotide probes immobilised on microspheres. Subsequently, the addition of streptavidin-PE buffer allows visualisation of the hybridisation pattern using the LUMINEX system.
Reference Values
With low-resolution typing, for each HLA gene two results are obtained, for example: HLA-DRB1*03 DRB1*13.
Diagnostic Algorithm:
Not applicable.
Turnaround Time:
20 working days.
Specimen information
Sample: Whole Blood
Tube: EDTA K3 4 ml Tube
Minimum volume: 500µl.
Stability:
- In refrigeration: 2 weeks.
- At -20ºC: from 2 weeks onwards.
Transport instructions: Preferably at room temperature
Reason for rejection: Clotted sample
Administrative information
BST Code: LRD2008
Test Description: Low-resolution HLA-DRB1 Typing
Synonyms: HLA-DRB1
Section: Histocompatibility and Immunogenetics
BST Rate: Check the updated rates here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).