HLA-DPB1 high resolution Typing

Clinical information

Diagnostic Utility:

High-resolution HLA typing allows us to determine the HLA typing at an allelic level (4 digits) of:

• Patients eligible for hematopoietic stem cell transplantation.
• Identical family members of patients eligible for hematopoietic stem cell transplantation.
• Voluntary donor registry.
• Expansion of HLA typing in voluntary donors.
• Registry and verification of umbilical cord blood units.
• Pre-transplant confirmation of donor and recipient.

Method:

The PCR-NGS technique (Long strategy and Short strategy) allows high-resolution HLA typing of class I and II HLA genes and non-classical HLA genes. This technique involves several stages: amplification of the HLA genes of interest by PCR, library preparation, sequencing, and result analysis. The Long strategy (long fragments) provides sequencing of the entire gene, while the Short strategy (short fragments) sequences exons 2, 3, and 4 of the HLA-A, -B, and -C genes, and exons 2 and 3 of the HLA-DRB1, -DQB1, -DPB1, -DQA1, and -DPA1 genes.

The PCR-SBT technique allows high-resolution HLA typing of an isolated HLA gene. This technique consists of two phases: amplification of the exons of the HLA genes of interest by PCR and sequencing reaction of the amplified products. Finally, the sequences are analysed by electrophoresis. Exons 2 and 3 of the HLA-DPB1 gene are sequenced.

Reference Values

With high-resolution typing, two results are obtained for each HLA gene, for example: HLA-DPB1*04:01 DPB1*17:01.

Diagnostic Algorithm:

Not applicable.

Turnaround Time:

The turnaround time for high-resolution HLA typing depends on the origin of the sample:

• Patient or family members of a patient eligible for hematopoietic stem cell transplantation (20 working days).
• Voluntary donor registry (20 working days).
• Expansion of HLA typing in voluntary donors (10 working days).
• Registry of umbilical cord blood units (20 working days).
• Verification of umbilical cord blood units (20 working days).
• Pre-transplant confirmation of donor and recipient (20 working days).

Specimen information

Sample: Whole Blood
Tube: 4 ml EDTA K3 Tube
Minimum volume: 500 µl.

Stability:

• In refrigeration: 2 weeks.
• At -20°C: from 2 weeks onwards.

Transport instructions: Preferably at room temperature

Reason for rejection: Clotted sample

Administrative information

BST Code: LRD2012
Test Description: High Resolution HLA-DPB1 Typing
Synonyms: HLA-DPB1 high.
Section: Histocompatibility and Immunogenetics
BST Rate: Check the updated rates here.

Profiles:

The High Resolution HLA-DPB1 test is included in the "High Resolution HLA Typing Complete Profile" (see table).

References

Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).