Genotype HLA associated with Behçet B51

Clinical information

Diagnostic Utility:

Studying the HLA genotype allows us to identify HLA antigens associated with diseases such as Behçet's disease (HLA-B*51).

Method:

PCR-SSO for the HLA-B locus.

Exons 2 and 3 of the HLA-B gene are amplified by PCR using biotinylated primers. The amplified products are then hybridised with specific oligonucleotide probes immobilised on microspheres. Subsequently, the addition of a streptavidin-PE buffer allows visualisation of the hybridisation pattern using the LUMINEX system.

Reference Values

The possible results are: positive for B*51 or negative for B*51.

Diagnostic Algorithm:

Not applicable.

Turnaround Time:

15 working days.

Specimen information

Sample: Whole blood
Tube: EDTA K3 4 ml tube
Minimum volume: 500 µl.

Stability:

• In refrigeration: 2 weeks.
• At -20°C: from 2 weeks onwards.

Transport instructions: Preferably at room temperature

Reason for rejection: Clotted sample.

Administrative information

BST Code: LRD2051
Test Description: HLA genotype associated with Behçet's disease (B51)
Synonyms: B51
Section: Histocompatibility and Immunogenetics
BST Fee: Check the updated fees here.
Profiles: Not applicable.

References

Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).