Clinical information
Diagnostic Utility:
Determining the HLA B*57:01 allele allows us to identify patients with hypersensitivity to Abacavir treatment.
Method:
Typing of the B*57 antigen using real-time PCR and high-resolution HLA-B typing using PCR-NGS.
Reference Values
In B*57 antigen typing, the possible results are: negative B*57 or positive B*57.
In high-resolution HLA-B typing, the possible results are: negative B*57:01 or positive B*57:01.
Diagnostic Algorithm:
Following the diagnostic algorithm, if the result of the real-time PCR is positive for B57, PCR-NGS is performed to determine if it is B*57:01.
Turnaround Time:
15 working days.
Specimen information
Sample: Whole Blood
Tube: EDTA K3 4 ml Tube
Minimum volume: 500µl.
Stability:
- In refrigeration: 2 weeks.
- At -20ºC: from 2 weeks onwards.
Transport Instructions: Preferably at room temperature
Reason for rejection: Clotted sample.
Administrative information
BST Code: LRD2056
Test Description: HLA-B*57:01
Synonyms: Not applicable.
Section: Histocompatibility and Immunogenetics
BST Fee: Check the updated fees here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).