Clinical information

Diagnostic Utility:

Determining the HLA B*57:01 allele allows us to identify patients with hypersensitivity to Abacavir treatment.

Method:

Typing of the B*57 antigen using real-time PCR and high-resolution HLA-B typing using PCR-NGS.

Reference Values

In B*57 antigen typing, the possible results are: negative B*57 or positive B*57.
In high-resolution HLA-B typing, the possible results are: negative B*57:01 or positive B*57:01.

Diagnostic Algorithm:

Following the diagnostic algorithm, if the result of the real-time PCR is positive for B57, PCR-NGS is performed to determine if it is B*57:01.

Turnaround Time:

15 working days.

Specimen information

Sample: Whole Blood

Tube: EDTA K3 4 ml Tube

Minimum volume: 500µl.

Stability:

  • In refrigeration: 2 weeks.
  • At -20ºC: from 2 weeks onwards.

Transport Instructions: Preferably at room temperature

Reason for rejection: Clotted sample.

Administrative information

BST Code: LRD2056
Test Description: HLA-B*57:01
Synonyms: Not applicable.
Section: Histocompatibility and Immunogenetics
BST Fee: Check the updated fees here.
Profiles: Not applicable.

References

Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).

Quality

BST holds ISO 9001, ISO 14001, and OSHAS 18001 quality certifications, as well as the European Excellence 500+ seal. BST is accredited by CAT, JACIE-FACT, FACT-NETCORD, and EFI, and complies with Good Manufacturing Practice (GMP) and Good Distribution Practice (GDP) guidelines.