Clinical information
Diagnostic Utility:
The search for anti-HLA antibodies allows us to detect the presence of anti-HLA class I and class II antibodies in:
- Patients candidates for hematopoietic stem cell transplantation.
- Patients candidates for solid organ transplantation.
- Studies related to transfusion: platelet refractoriness and suspected Transfusion-Related Acute Lung Injury (TRALI).
Method:
Solid-phase assay (Luminex).
The serum to be analyzed is incubated with microspheres that present HLA antigens on their surface. There are 7 different groups of microspheres for HLA class I and 5 groups for HLA class II. A human anti-IgG antibody conjugated to phycoerythrin is added, allowing the visualization of the binding of anti-HLA antibodies present in the serum using the LUMINEX device.
Reference Values
In the screening, the presence or absence of anti-HLA antibodies is determined. The possible results are: Negative or Positive for anti-HLA class I and/or II antibodies. A sample is considered positive for anti-HLA class I antibodies if at least one of the 7 groups of HLA class I microspheres is positive. On the other hand, a sample is considered positive for anti-HLA class II antibodies if at least one of the 5 groups of HLA class II microspheres is positive.
Diagnostic Algorithm:
Following the diagnostic algorithm, if the result of the search for anti-HLA antibodies is positive (for class I and/or II), the following tests can be generated:
| Code | Test Name |
|---|---|
| LRD20017 | Identification of anti-HLA class I antibodies |
| LRD20059 | Identification of anti-HLA class II antibodies |
Turnaround Time:
15 working days.
Specimen information
Sample: Serum
Tube: 8.5 ml separator gel tube
Minimum volume:
- Blood: 500µl.
- Serum: 100 µl.
Stability:
- In refrigeration: 2 days.
- At -20ºC: from day 3 onwards.
Transport instructions:
If it is a blood sample preferably at room temperature.
If it is a serum sample preferably refrigerated.
Reason for rejection: Highly hemolyzed sample
Administrative information
BST Code: LRD2016
Test Description: Anti-HLA antibodies research
Synonyms: Not applicable
Section: Histocompatibility and Immunogenetics
BST Fee: Check the updated fees here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).
Quality
BST holds ISO 9001, ISO 14001, and OSHAS 18001 quality certifications, as well as the European Excellence 500+ seal. BST is accredited by CAT, JACIE-FACT, FACT-NETCORD, and EFI, and complies with Good Manufacturing Practice (GMP) and Good Distribution Practice (GDP) guidelines.