Clinical information
Diagnostic utility:
- This determination is useful in typing patients or donors where confirming the presence or absence of this antigen is of interest.
- There are different techniques for typing HPA antigens, with molecular biology techniques currently being the most widely used.
Method:
Determination of HPA-5b genotype
Prior to determining the genotype, DNA extraction must be performed from a blood sample in EDTA.
Platelet genotype study HPA-5b is carried out using the PCR SSP technique, with specific primers for the HPA-5b allele in a uniplex.
Results:
The results are:
- Positive
- Negative
Response time:
10 working days
Specimen information
Sample: Peripheral blood in EDTA
Sample volume: 1 tube of 5 ml of EDTA K3
Stability: At room temperature or at 4°C: 7 days
Transport instructions: Preferably at room temperature
Reason for rejection: Highly hemolyzed sample
Administrative information
BST Code: 0609
Test Description: Platelet Antigen HPA-5b
Synonyms: --
Section: Immunohematology
BST Rate: Check the updated rates here.
Profiles: It is not included in any profile
References
G.Cavanagh, A. N. Dunn, C. E. Chapman i P. Metcalfe. " HPA genotyping by PCR sequence-specific priming (PCR-SSP): a streamlined method for rapid routine investigations". Transfusion Medicine, 7: 41-45 (1997).