Phenotypic Characterisation of Platelets

Code: CEL018

Type: Control de qualitat de components sanguinis Control de qualitat de teràpia cel•lular

Clinical information

Diagnostic Utility:

This test evaluates the phenotype of platelets from different types of samples such as platelet pools, platelet-rich plasma (PRP), platelet apheresis, etc. Specifically, it assesses the expression of different membrane proteins: CD41a (GP IIb), CD49e (integrin α5), CD42b (GP Ibα), CD36 (GP IV) as well as the activation markers CD62P (P-selectin), CD63, and Annexin V.

Method:

Flow Cytometry.

Reference Values

The results are quantitative (expressed in percentage) and may vary depending on the type of sample analyzed or whether they come from a healthy donor or a patient.

Diagnostic Algorithm:

Not applicable.

Turnaround Time:

1 day.

Specimen information

Sample: Platelet pools, platelet-rich plasma (PRPs), plateletpheresis, etc.
Tube: Polystyrene or glass tube without additive
Minimum volume: 500 µL

Stability:

Fresh sample: 1 day
Thawed samples: 1 hour

Transport instructions: Room temperature.

Reason for rejection: Sample with clots.

Administrative information

BST Code: CEL018
Test Description: Phenotypic characterization of platelets.
Synonyms: Phen platelets
Section: Cell Laboratory
BST Rate: Check the updated rates here.

Profiles:

This test is not included in any profile.

References

Pasalic L et al; Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood; Methods Mol Biol. 2017;1646:369-389.

Sandgren P et al; Storage of buffy-coat-derived platelets in additive solutions at 4 degrees C and 22 degrees C: flow cytometry analysis of platelet glycoprotein expression. Vox Sang. 2007 Jul;93(1):27-36.