Clinical information
Diagnostic Utility:
- Studying the expression of platelet membrane glycoproteins is a diagnostic test requested in cases of suspected deficiency of any of the GPs, for example in the suspicion of Bernard-Soulier syndrome (deficiency of GP Ib/IX/V) and in the suspicion of Glanzmann thrombasthenia (deficiency of GP IIb/IIIa).
- These are rare inherited disorders that manifest through bleeding complications.
Method:
Immunofluorescence technique, with flow cytometry reading.
The patient's platelets and control platelets are incubated with monoclonal antibodies against GPs IIb/IIIa, Ia/IIa, and Ib/IX, and the intensity of the expression of different platelet membrane GPs is read by flow cytometry.
Results:
The results of the study are:
- The expression of glycoproteins IIb/IIIa, Ia/IIa, and Ib on the platelet membrane, analyzed by Flow Cytometry, shows levels comparable to those of a normal control.
- A deficiency in the expression of some platelet membrane glycoproteins is observed.
Precautions:
Response Time:
4 working days
Specimen information
Sample: Peripheral Blood
Tubes in adult patients:
- 2 tubes of 10 ml of EDTA K3
Tubes in pediatric patients:
- 1 tube of 10 ml EDTA or the maximum possible volume of EDTA according to the child's age
Stability: At room temperature or at 4°C: 3 days
Transport instructions: Preferably at room temperature
Reason for rejection: Highly hemolyzed sample
Administrative information
BST Code: 4811
Test Description: Platelet Membrane Glycoproteins
Synonyms:
Section: Immunohematology
BST Rate: Check the updated rates here.
Profiles:
Test 4811 Study of Platelet Membrane Glycoproteins is not included in any profile.
References
Paul Harrison, Ian Mackie, Andrew Mumford, Carol Briggs,, Ri Liesner, Mark Winter, Sam Machin and British Committee for Standards in Haematology. Guidelines for the laboratory investigation of heritable disorders of platelet function. British Journal of Haematology 2011; 155, 30-44