Quantification/viability of CD34 and CD45 ISHAGE

Clinical information

Diagnostic Utility:

This test quantifies:

• cells positive for the CD34 membrane receptor (hematopoietic progenitors)
• cells positive for CD45 (common leukocyte antigen)

and determines cellular viability. It is a determination used as quality control for units of umbilical cord blood and products for hematopoietic progenitor transplantation.

Method: 

Flow Cytometry.

Reference Values

The results are quantitative. CD34 and CD45 concentrations can vary significantly depending on the type of sample/product and whether they come from a healthy donor or a patient. As for cellular viability, the expected values are as follows:

Diagnostic Algorithm:

Not applicable.

Turnaround Time:

1 day.

Specimen information

Sample: Peripheral blood, umbilical cord blood, bone marrow, apheresis.
Tube: EDTA K3 tube (peripheral blood) or tube without additive (anticoagulated samples).
Minimum volume: 100 µL

Stability:

• At room temperature: 1 day
• In refrigeration: 1 day
• Cryopreserved between -156^oC and -196^oC: 20 years

Transport instructions: Preferably peripheral blood at room temperature; umbilical cord blood, bone marrow, apheresis refrigerated.

Reason for rejection: Sample highly hemolyzed or with clots.

Administrative information

BST Code: CEL007
Test Description: Quantification and viability of CD34 and CD45 cells by the ISHAGE method.
Synonyms: R/V CD34, ISHAGE
Section: Cellular Laboratory
BST Rates: Check the updated rates here.

Profiles:

Test CEL007 Quantification/viability of CD34 and CD45 cells by ISHAGE can be requested alone. It is also performed within the following profiles:

References

Sutherland R;  Enumeration of CD34+ Cells by Flow Cytometry; Cellular Therapy: Principles, Methods, and Regulations, Chapter 56, 2nd Edition, AABB, 2016.